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Laser Assisted Hatching (LAH) is a laboratory technique used during in vitro fertilization (IVF) to help an embryo implant more easily into the uterine lining. It involves using a precision laser to create a small opening in the outer shell of the embryo, known as the zona pellucida. In early development, embryos are surrounded by the zona pellucida, a protective layer that the embryo must "hatch" from before it can implant in the uterus. In some cases, especially in older patients or when embryos have been frozen and thawed, this shell can become thicker or harder, making natural hatching more difficult. LAH helps overcome this barrier.
Embryo Development: The embryo is typically cultured to the day 3 (cleavage stage) or day 5 (blastocyst stage).
Laser Application: A highly focused laser is used to thin or create a small hole in the zona pellucida while the embryo is held still under a microscope.
Embryo Transfer: The treated embryo is then transferred to the uterus, where the opened zona may help it hatch and implant more successfully.
Women over age 35.
Patients with previous failed IVF cycles.
Embryos with a thick zona pellucida.
Use of frozen-thawed embryos.
Cases with poor embryo implantation history.
May improve implantation and pregnancy rates in select patients.
Increases the likelihood of successful hatching, a critical step before implantation.
Precision and safety: Laser provides high control with minimal damage to the embryo.
Not needed for all IVF patients; most embryos hatch on their own.
While generally safe, LAH involves manipulation of the embryo and should only be done by experienced embryologists.
It does not guarantee implantation or pregnancy.